Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_9_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_9_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:55 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 47573665
Paired-end alignments with a unique best hit 29242056
Pairs without alignments under any condition 11968391
Pairs that did not map uniquely 6363218
Genomic sequence context not extractable (edges of chromosomes) 3271

Cytosine Methylation

Total C's analysed 1019347442
Methylated C's in CpG context 14622783
Methylated C's in CHG context 853231
Methylated C's in CHH context 3562620
Methylated C's in Unknown context 36997
Unmethylated C's in CpG context 116851567
Unmethylated C's in CHG context 178719809
Unmethylated C's in CHH context 704737432
Unmethylated C's in Unknown context 2493127
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7340864 original top strand
CTOT 7275172 complementary to original top strand
CTOB 7277422 complementary to original bottom strand
OB 7345327 original bottom strand

Deduplication

Alignments analysed 29238785
Unique alignments 25551910
Duplicates removed 3686875
Duplicated alignments were found at 3203203 different positions

Cytosine Methylation after Extraction

Total C's analysed 585196152
Methylated C's in CpG context 8185845
Methylated C's in CHG context 483963
Methylated C's in CHH context 2033084
Unmethylated C's in CpG context 66601575
Unmethylated C's in CHG context 101618372
Unmethylated C's in CHH context 406273313
Percentage methylation (CpG context) 10.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot