Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_8_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_8_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:55 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 42336845
Paired-end alignments with a unique best hit 26007499
Pairs without alignments under any condition 11049243
Pairs that did not map uniquely 5280103
Genomic sequence context not extractable (edges of chromosomes) 3208

Cytosine Methylation

Total C's analysed 917080681
Methylated C's in CpG context 12681846
Methylated C's in CHG context 798448
Methylated C's in CHH context 3381738
Methylated C's in Unknown context 32857
Unmethylated C's in CpG context 104398980
Unmethylated C's in CHG context 159572763
Unmethylated C's in CHH context 636246906
Unmethylated C's in Unknown context 2250422
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6495653 original top strand
CTOT 6499419 complementary to original top strand
CTOB 6502055 complementary to original bottom strand
OB 6507164 original bottom strand

Deduplication

Alignments analysed 26004291
Unique alignments 23158534
Duplicates removed 2845757
Duplicated alignments were found at 2515230 different positions

Cytosine Methylation after Extraction

Total C's analysed 558395155
Methylated C's in CpG context 7565296
Methylated C's in CHG context 480801
Methylated C's in CHH context 2036378
Unmethylated C's in CpG context 63221600
Unmethylated C's in CHG context 96372135
Unmethylated C's in CHH context 388718945
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot