Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_7_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_7_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 81572749
Paired-end alignments with a unique best hit 49808265
Pairs without alignments under any condition 22049639
Pairs that did not map uniquely 9714845
Genomic sequence context not extractable (edges of chromosomes) 6088

Cytosine Methylation

Total C's analysed 1760829695
Methylated C's in CpG context 23877029
Methylated C's in CHG context 1490398
Methylated C's in CHH context 6273620
Methylated C's in Unknown context 60584
Unmethylated C's in CpG context 198654689
Unmethylated C's in CHG context 303395937
Unmethylated C's in CHH context 1227138022
Unmethylated C's in Unknown context 4448566
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12445023 original top strand
CTOT 12446983 complementary to original top strand
CTOB 12454757 complementary to original bottom strand
OB 12455414 original bottom strand

Deduplication

Alignments analysed 49802177
Unique alignments 44057955
Duplicates removed 5744222
Duplicated alignments were found at 5009585 different positions

Cytosine Methylation after Extraction

Total C's analysed 1077845907
Methylated C's in CpG context 14313577
Methylated C's in CHG context 900907
Methylated C's in CHH context 3799355
Unmethylated C's in CpG context 120868908
Unmethylated C's in CHG context 184099247
Unmethylated C's in CHH context 753863913
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot