Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_6_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_6_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 53174813
Paired-end alignments with a unique best hit 32883973
Pairs without alignments under any condition 13870904
Pairs that did not map uniquely 6419936
Genomic sequence context not extractable (edges of chromosomes) 3856

Cytosine Methylation

Total C's analysed 1163306727
Methylated C's in CpG context 15519079
Methylated C's in CHG context 987998
Methylated C's in CHH context 4159215
Methylated C's in Unknown context 39191
Unmethylated C's in CpG context 131573132
Unmethylated C's in CHG context 201241492
Unmethylated C's in CHH context 809825811
Unmethylated C's in Unknown context 2904130
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8232092 original top strand
CTOT 8215780 complementary to original top strand
CTOB 8209212 complementary to original bottom strand
OB 8223033 original bottom strand

Deduplication

Alignments analysed 32880117
Unique alignments 28863413
Duplicates removed 4016704
Duplicated alignments were found at 3502031 different positions

Cytosine Methylation after Extraction

Total C's analysed 700942212
Methylated C's in CpG context 9171379
Methylated C's in CHG context 588112
Methylated C's in CHH context 2480715
Unmethylated C's in CpG context 78889570
Unmethylated C's in CHG context 120317877
Unmethylated C's in CHH context 489494559
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot