Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_5_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 44083656
Paired-end alignments with a unique best hit 27062582
Pairs without alignments under any condition 11774109
Pairs that did not map uniquely 5246965
Genomic sequence context not extractable (edges of chromosomes) 3097

Cytosine Methylation

Total C's analysed 958806516
Methylated C's in CpG context 12849444
Methylated C's in CHG context 809839
Methylated C's in CHH context 3383314
Methylated C's in Unknown context 32893
Unmethylated C's in CpG context 108112391
Unmethylated C's in CHG context 165705948
Unmethylated C's in CHH context 667945580
Unmethylated C's in Unknown context 2393863
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6715808 original top strand
CTOT 6808129 complementary to original top strand
CTOB 6813222 complementary to original bottom strand
OB 6722326 original bottom strand

Deduplication

Alignments analysed 27059485
Unique alignments 24112850
Duplicates removed 2946635
Duplicated alignments were found at 2601507 different positions

Cytosine Methylation after Extraction

Total C's analysed 589371788
Methylated C's in CpG context 7735787
Methylated C's in CHG context 493412
Methylated C's in CHH context 2060645
Unmethylated C's in CpG context 66114577
Unmethylated C's in CHG context 101010033
Unmethylated C's in CHH context 411957334
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot