Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_4_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_4_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 49306248
Paired-end alignments with a unique best hit 30294123
Pairs without alignments under any condition 13031821
Pairs that did not map uniquely 5980304
Genomic sequence context not extractable (edges of chromosomes) 3392

Cytosine Methylation

Total C's analysed 1072084783
Methylated C's in CpG context 13911788
Methylated C's in CHG context 863058
Methylated C's in CHH context 3571494
Methylated C's in Unknown context 35667
Unmethylated C's in CpG context 121521063
Unmethylated C's in CHG context 184074892
Unmethylated C's in CHH context 748142488
Unmethylated C's in Unknown context 2695306
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7582775 original top strand
CTOT 7560867 complementary to original top strand
CTOB 7564330 complementary to original bottom strand
OB 7582759 original bottom strand

Deduplication

Alignments analysed 30290731
Unique alignments 26727717
Duplicates removed 3563014
Duplicated alignments were found at 3137361 different positions

Cytosine Methylation after Extraction

Total C's analysed 647545749
Methylated C's in CpG context 8230533
Methylated C's in CHG context 516089
Methylated C's in CHH context 2143339
Unmethylated C's in CpG context 73038669
Unmethylated C's in CHG context 110297480
Unmethylated C's in CHH context 453319639
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot