Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_3_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_3_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 38913609
Paired-end alignments with a unique best hit 23805179
Pairs without alignments under any condition 10343037
Pairs that did not map uniquely 4765393
Genomic sequence context not extractable (edges of chromosomes) 3159

Cytosine Methylation

Total C's analysed 841165030
Methylated C's in CpG context 11246015
Methylated C's in CHG context 700201
Methylated C's in CHH context 2945412
Methylated C's in Unknown context 28949
Unmethylated C's in CpG context 95610735
Unmethylated C's in CHG context 145538838
Unmethylated C's in CHH context 585123829
Unmethylated C's in Unknown context 2102258
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5932391 original top strand
CTOT 5956670 complementary to original top strand
CTOB 5970104 complementary to original bottom strand
OB 5942855 original bottom strand

Deduplication

Alignments analysed 23802020
Unique alignments 21326564
Duplicates removed 2475456
Duplicated alignments were found at 2188277 different positions

Cytosine Methylation after Extraction

Total C's analysed 519734226
Methylated C's in CpG context 6812889
Methylated C's in CHG context 427744
Methylated C's in CHH context 1799739
Unmethylated C's in CpG context 58763328
Unmethylated C's in CHG context 89213203
Unmethylated C's in CHH context 362717323
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot