Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_2_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_2_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 55025914
Paired-end alignments with a unique best hit 33615008
Pairs without alignments under any condition 14806238
Pairs that did not map uniquely 6604668
Genomic sequence context not extractable (edges of chromosomes) 4620

Cytosine Methylation

Total C's analysed 1197280356
Methylated C's in CpG context 15775590
Methylated C's in CHG context 1001189
Methylated C's in CHH context 4199273
Methylated C's in Unknown context 39115
Unmethylated C's in CpG context 135568039
Unmethylated C's in CHG context 206239669
Unmethylated C's in CHH context 834496596
Unmethylated C's in Unknown context 3014832
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8415908 original top strand
CTOT 8397912 complementary to original top strand
CTOB 8393898 complementary to original bottom strand
OB 8402670 original bottom strand

Deduplication

Alignments analysed 33610388
Unique alignments 29344794
Duplicates removed 4265594
Duplicated alignments were found at 3696181 different positions

Cytosine Methylation after Extraction

Total C's analysed 734681415
Methylated C's in CpG context 9492033
Methylated C's in CHG context 606030
Methylated C's in CHH context 2545972
Unmethylated C's in CpG context 82654442
Unmethylated C's in CHG context 125509718
Unmethylated C's in CHH context 513873220
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot