Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_24_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_24_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:54 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 52244045
Paired-end alignments with a unique best hit 32479152
Pairs without alignments under any condition 13303250
Pairs that did not map uniquely 6461643
Genomic sequence context not extractable (edges of chromosomes) 4026

Cytosine Methylation

Total C's analysed 1152358324
Methylated C's in CpG context 15525678
Methylated C's in CHG context 990475
Methylated C's in CHH context 4125982
Methylated C's in Unknown context 38120
Unmethylated C's in CpG context 131489526
Unmethylated C's in CHG context 201060109
Unmethylated C's in CHH context 799166554
Unmethylated C's in Unknown context 2835426
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8114827 original top strand
CTOT 8116900 complementary to original top strand
CTOB 8121612 complementary to original bottom strand
OB 8121787 original bottom strand

Deduplication

Alignments analysed 32475126
Unique alignments 28564805
Duplicates removed 3910321
Duplicated alignments were found at 3411445 different positions

Cytosine Methylation after Extraction

Total C's analysed 696581319
Methylated C's in CpG context 9199601
Methylated C's in CHG context 591767
Methylated C's in CHH context 2471218
Unmethylated C's in CpG context 78991176
Unmethylated C's in CHG context 120496784
Unmethylated C's in CHH context 484830773
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot