Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_23_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_23_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:53 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 39902509
Paired-end alignments with a unique best hit 24721544
Pairs without alignments under any condition 10161807
Pairs that did not map uniquely 5019158
Genomic sequence context not extractable (edges of chromosomes) 2976

Cytosine Methylation

Total C's analysed 871034956
Methylated C's in CpG context 11700596
Methylated C's in CHG context 750578
Methylated C's in CHH context 3129842
Methylated C's in Unknown context 29479
Unmethylated C's in CpG context 99735567
Unmethylated C's in CHG context 151894105
Unmethylated C's in CHH context 603824268
Unmethylated C's in Unknown context 2139223
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6208454 original top strand
CTOT 6151521 complementary to original top strand
CTOB 6155092 complementary to original bottom strand
OB 6203501 original bottom strand

Deduplication

Alignments analysed 24718568
Unique alignments 21853588
Duplicates removed 2864980
Duplicated alignments were found at 2528512 different positions

Cytosine Methylation after Extraction

Total C's analysed 523762933
Methylated C's in CpG context 6879279
Methylated C's in CHG context 445302
Methylated C's in CHH context 1864072
Unmethylated C's in CpG context 59597776
Unmethylated C's in CHG context 90544405
Unmethylated C's in CHH context 364432099
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot