Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_22_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_22_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:53 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 47019646
Paired-end alignments with a unique best hit 29164293
Pairs without alignments under any condition 12055540
Pairs that did not map uniquely 5799813
Genomic sequence context not extractable (edges of chromosomes) 3674

Cytosine Methylation

Total C's analysed 1035254685
Methylated C's in CpG context 14078388
Methylated C's in CHG context 898075
Methylated C's in CHH context 3733158
Methylated C's in Unknown context 33672
Unmethylated C's in CpG context 118463031
Unmethylated C's in CHG context 181180018
Unmethylated C's in CHH context 716902015
Unmethylated C's in Unknown context 2536356
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7261504 original top strand
CTOT 7309226 complementary to original top strand
CTOB 7317228 complementary to original bottom strand
OB 7272661 original bottom strand

Deduplication

Alignments analysed 29160619
Unique alignments 25736671
Duplicates removed 3423948
Duplicated alignments were found at 3003485 different positions

Cytosine Methylation after Extraction

Total C's analysed 628767897
Methylated C's in CpG context 8357109
Methylated C's in CHG context 538491
Methylated C's in CHH context 2239763
Unmethylated C's in CpG context 71452883
Unmethylated C's in CHG context 109045767
Unmethylated C's in CHH context 437133884
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot