Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_21_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_21_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:53 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 50457301
Paired-end alignments with a unique best hit 30932612
Pairs without alignments under any condition 13479625
Pairs that did not map uniquely 6045064
Genomic sequence context not extractable (edges of chromosomes) 3820

Cytosine Methylation

Total C's analysed 1092232309
Methylated C's in CpG context 14831994
Methylated C's in CHG context 933395
Methylated C's in CHH context 3877033
Methylated C's in Unknown context 36631
Unmethylated C's in CpG context 124348339
Unmethylated C's in CHG context 189579802
Unmethylated C's in CHH context 758661746
Unmethylated C's in Unknown context 2715605
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7737803 original top strand
CTOT 7729999 complementary to original top strand
CTOB 7725591 complementary to original bottom strand
OB 7735399 original bottom strand

Deduplication

Alignments analysed 30928792
Unique alignments 27320626
Duplicates removed 3608166
Duplicated alignments were found at 3172581 different positions

Cytosine Methylation after Extraction

Total C's analysed 671295159
Methylated C's in CpG context 8897366
Methylated C's in CHG context 566089
Methylated C's in CHH context 2357111
Unmethylated C's in CpG context 75903501
Unmethylated C's in CHG context 115427065
Unmethylated C's in CHH context 468144027
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot