Bismark report for: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Hawes/data/Cg-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53390071 Number of paired-end alignments with a unique best hit: 32489129 Mapping efficiency: 60.9% Sequence pairs with no alignments under any condition: 14599336 Sequence pairs did not map uniquely: 6301606 Sequence pairs which were discarded because genomic sequence could not be extracted: 3848 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7817880 ((converted) top strand) GA/CT/CT: 8415796 (complementary to (converted) top strand) GA/CT/GA: 8422205 (complementary to (converted) bottom strand) CT/GA/GA: 7829400 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1148855238 Total methylated C's in CpG context: 15446101 Total methylated C's in CHG context: 997641 Total methylated C's in CHH context: 4136554 Total methylated C's in Unknown context: 42585 Total unmethylated C's in CpG context: 130764316 Total unmethylated C's in CHG context: 199305094 Total unmethylated C's in CHH context: 798205532 Total unmethylated C's in Unknown context: 2810117 C methylated in CpG context: 10.6% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.5% Bismark completed in 0d 5h 12m 50s