Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:53 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 53390071
Paired-end alignments with a unique best hit 32489129
Pairs without alignments under any condition 14599336
Pairs that did not map uniquely 6301606
Genomic sequence context not extractable (edges of chromosomes) 3848

Cytosine Methylation

Total C's analysed 1148855238
Methylated C's in CpG context 15446101
Methylated C's in CHG context 997641
Methylated C's in CHH context 4136554
Methylated C's in Unknown context 42585
Unmethylated C's in CpG context 130764316
Unmethylated C's in CHG context 199305094
Unmethylated C's in CHH context 798205532
Unmethylated C's in Unknown context 2810117
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7817880 original top strand
CTOT 8415796 complementary to original top strand
CTOB 8422205 complementary to original bottom strand
OB 7829400 original bottom strand

Deduplication

Alignments analysed 32485281
Unique alignments 28553777
Duplicates removed 3931504
Duplicated alignments were found at 3449859 different positions

Cytosine Methylation after Extraction

Total C's analysed 704746106
Methylated C's in CpG context 9253793
Methylated C's in CHG context 608123
Methylated C's in CHH context 2523251
Unmethylated C's in CpG context 79760722
Unmethylated C's in CHG context 121187516
Unmethylated C's in CHH context 491412701
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot