Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_1_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_1_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:53 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 50993451
Paired-end alignments with a unique best hit 31687838
Pairs without alignments under any condition 12773379
Pairs that did not map uniquely 6532234
Genomic sequence context not extractable (edges of chromosomes) 3518

Cytosine Methylation

Total C's analysed 1115774564
Methylated C's in CpG context 13928703
Methylated C's in CHG context 914564
Methylated C's in CHH context 3838735
Methylated C's in Unknown context 35679
Unmethylated C's in CpG context 127975064
Unmethylated C's in CHG context 194760133
Unmethylated C's in CHH context 774357365
Unmethylated C's in Unknown context 2721469
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7969382 original top strand
CTOT 7870081 complementary to original top strand
CTOB 7871018 complementary to original bottom strand
OB 7973839 original bottom strand

Deduplication

Alignments analysed 31684320
Unique alignments 27716181
Duplicates removed 3968139
Duplicated alignments were found at 3451332 different positions

Cytosine Methylation after Extraction

Total C's analysed 656185088
Methylated C's in CpG context 8019564
Methylated C's in CHG context 530436
Methylated C's in CHH context 2233158
Unmethylated C's in CpG context 74703497
Unmethylated C's in CHG context 113553021
Unmethylated C's in CHH context 457145412
Percentage methylation (CpG context) 9.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot