Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_19_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_19_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:52 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 48641567
Paired-end alignments with a unique best hit 30051443
Pairs without alignments under any condition 12637251
Pairs that did not map uniquely 5952873
Genomic sequence context not extractable (edges of chromosomes) 3637

Cytosine Methylation

Total C's analysed 1058145249
Methylated C's in CpG context 14132667
Methylated C's in CHG context 903036
Methylated C's in CHH context 3797661
Methylated C's in Unknown context 34754
Unmethylated C's in CpG context 120516438
Unmethylated C's in CHG context 184031862
Unmethylated C's in CHH context 734763585
Unmethylated C's in Unknown context 2613391
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7510668 original top strand
CTOT 7514424 complementary to original top strand
CTOB 7516518 complementary to original bottom strand
OB 7506196 original bottom strand

Deduplication

Alignments analysed 30047806
Unique alignments 26814938
Duplicates removed 3232868
Duplicated alignments were found at 2852110 different positions

Cytosine Methylation after Extraction

Total C's analysed 652076966
Methylated C's in CpG context 8508318
Methylated C's in CHG context 548270
Methylated C's in CHH context 2309048
Unmethylated C's in CpG context 73797909
Unmethylated C's in CHG context 112407205
Unmethylated C's in CHH context 454506216
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot