Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_17_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_17_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:52 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 57429743
Paired-end alignments with a unique best hit 35424111
Pairs without alignments under any condition 14427268
Pairs that did not map uniquely 7578364
Genomic sequence context not extractable (edges of chromosomes) 4604

Cytosine Methylation

Total C's analysed 1254819599
Methylated C's in CpG context 17184173
Methylated C's in CHG context 1093647
Methylated C's in CHH context 4560361
Methylated C's in Unknown context 41487
Unmethylated C's in CpG context 144351881
Unmethylated C's in CHG context 219807459
Unmethylated C's in CHH context 867822078
Unmethylated C's in Unknown context 3050381
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8830397 original top strand
CTOT 8873766 complementary to original top strand
CTOB 8878358 complementary to original bottom strand
OB 8836986 original bottom strand

Deduplication

Alignments analysed 35419507
Unique alignments 30969523
Duplicates removed 4449984
Duplicated alignments were found at 3833712 different positions

Cytosine Methylation after Extraction

Total C's analysed 750665344
Methylated C's in CpG context 10065889
Methylated C's in CHG context 645928
Methylated C's in CHH context 2695248
Unmethylated C's in CpG context 85600717
Unmethylated C's in CHG context 130302636
Unmethylated C's in CHH context 521354926
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot