Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_16_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_16_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:52 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 50386602
Paired-end alignments with a unique best hit 31285722
Pairs without alignments under any condition 12561301
Pairs that did not map uniquely 6539579
Genomic sequence context not extractable (edges of chromosomes) 3703

Cytosine Methylation

Total C's analysed 1092880939
Methylated C's in CpG context 15057029
Methylated C's in CHG context 925777
Methylated C's in CHH context 3855517
Methylated C's in Unknown context 38274
Unmethylated C's in CpG context 124769225
Unmethylated C's in CHG context 190492163
Unmethylated C's in CHH context 757781228
Unmethylated C's in Unknown context 2679204
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7807771 original top strand
CTOT 7825361 complementary to original top strand
CTOB 7833818 complementary to original bottom strand
OB 7815069 original bottom strand

Deduplication

Alignments analysed 31282019
Unique alignments 27410458
Duplicates removed 3871561
Duplicated alignments were found at 3380110 different positions

Cytosine Methylation after Extraction

Total C's analysed 634507438
Methylated C's in CpG context 8550473
Methylated C's in CHG context 530647
Methylated C's in CHH context 2219073
Unmethylated C's in CpG context 71915952
Unmethylated C's in CHG context 109601187
Unmethylated C's in CHH context 441690106
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot