Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_15_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_15_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:51 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 58435477
Paired-end alignments with a unique best hit 36054469
Pairs without alignments under any condition 15375411
Pairs that did not map uniquely 7005597
Genomic sequence context not extractable (edges of chromosomes) 4286

Cytosine Methylation

Total C's analysed 1273183529
Methylated C's in CpG context 18451109
Methylated C's in CHG context 1105933
Methylated C's in CHH context 4644531
Methylated C's in Unknown context 45447
Unmethylated C's in CpG context 143403182
Unmethylated C's in CHG context 220305751
Unmethylated C's in CHH context 885273023
Unmethylated C's in Unknown context 3176983
Percentage methylation (CpG context) 11.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 9019232 original top strand
CTOT 9001460 complementary to original top strand
CTOB 9007257 complementary to original bottom strand
OB 9022234 original bottom strand

Deduplication

Alignments analysed 36050183
Unique alignments 31726902
Duplicates removed 4323281
Duplicated alignments were found at 3784019 different positions

Cytosine Methylation after Extraction

Total C's analysed 771512030
Methylated C's in CpG context 10932689
Methylated C's in CHG context 661769
Methylated C's in CHH context 2785392
Unmethylated C's in CpG context 86391470
Unmethylated C's in CHG context 132324684
Unmethylated C's in CHH context 538416026
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot