Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_14_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_14_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:51 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 36340861
Paired-end alignments with a unique best hit 22515262
Pairs without alignments under any condition 9464680
Pairs that did not map uniquely 4360919
Genomic sequence context not extractable (edges of chromosomes) 2364

Cytosine Methylation

Total C's analysed 793161390
Methylated C's in CpG context 12139072
Methylated C's in CHG context 705306
Methylated C's in CHH context 2907256
Methylated C's in Unknown context 29254
Unmethylated C's in CpG context 88512868
Unmethylated C's in CHG context 137509658
Unmethylated C's in CHH context 551387230
Unmethylated C's in Unknown context 1957994
Percentage methylation (CpG context) 12.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5605235 original top strand
CTOT 5650276 complementary to original top strand
CTOB 5650126 complementary to original bottom strand
OB 5607261 original bottom strand

Deduplication

Alignments analysed 22512898
Unique alignments 20107876
Duplicates removed 2405022
Duplicated alignments were found at 2133883 different positions

Cytosine Methylation after Extraction

Total C's analysed 486682336
Methylated C's in CpG context 7278291
Methylated C's in CHG context 427899
Methylated C's in CHH context 1764939
Unmethylated C's in CpG context 54042726
Unmethylated C's in CHG context 83650906
Unmethylated C's in CHH context 339517575
Percentage methylation (CpG context) 11.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot