Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:51 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 53303436
Paired-end alignments with a unique best hit 32764601
Pairs without alignments under any condition 14153632
Pairs that did not map uniquely 6385203
Genomic sequence context not extractable (edges of chromosomes) 4241

Cytosine Methylation

Total C's analysed 1160796741
Methylated C's in CpG context 15711645
Methylated C's in CHG context 993596
Methylated C's in CHH context 4173551
Methylated C's in Unknown context 38921
Unmethylated C's in CpG context 131370005
Unmethylated C's in CHG context 200977866
Unmethylated C's in CHH context 807570078
Unmethylated C's in Unknown context 2893967
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8187428 original top strand
CTOT 8184122 complementary to original top strand
CTOB 8194850 complementary to original bottom strand
OB 8193960 original bottom strand

Deduplication

Alignments analysed 32760360
Unique alignments 28778856
Duplicates removed 3981504
Duplicated alignments were found at 3482306 different positions

Cytosine Methylation after Extraction

Total C's analysed 708901527
Methylated C's in CpG context 9391702
Methylated C's in CHG context 598774
Methylated C's in CHH context 2518190
Unmethylated C's in CpG context 79696757
Unmethylated C's in CHG context 121675328
Unmethylated C's in CHH context 495020776
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot