Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_12_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_12_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:51 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 46849571
Paired-end alignments with a unique best hit 28948214
Pairs without alignments under any condition 12337394
Pairs that did not map uniquely 5563963
Genomic sequence context not extractable (edges of chromosomes) 3041

Cytosine Methylation

Total C's analysed 1014644269
Methylated C's in CpG context 12554888
Methylated C's in CHG context 808424
Methylated C's in CHH context 3407582
Methylated C's in Unknown context 32537
Unmethylated C's in CpG context 114031376
Unmethylated C's in CHG context 171147785
Unmethylated C's in CHH context 712694214
Unmethylated C's in Unknown context 2609786
Percentage methylation (CpG context) 9.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7211843 original top strand
CTOT 7277285 complementary to original top strand
CTOB 7252472 complementary to original bottom strand
OB 7203573 original bottom strand

Deduplication

Alignments analysed 28945173
Unique alignments 25658573
Duplicates removed 3286600
Duplicated alignments were found at 2908084 different positions

Cytosine Methylation after Extraction

Total C's analysed 612809937
Methylated C's in CpG context 7396838
Methylated C's in CHG context 482709
Methylated C's in CHH context 2040503
Unmethylated C's in CpG context 68440003
Unmethylated C's in CHG context 102458475
Unmethylated C's in CHH context 431991409
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot