Bismark report for: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Hawes/data/Cg-genome/Crassostrea_gigas.oyster_v9.dna_sm.toplevel/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 56063348 Number of paired-end alignments with a unique best hit: 34270614 Mapping efficiency: 61.1% Sequence pairs with no alignments under any condition: 14832830 Sequence pairs did not map uniquely: 6959904 Sequence pairs which were discarded because genomic sequence could not be extracted: 4580 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8553748 ((converted) top strand) GA/CT/CT: 8577936 (complementary to (converted) top strand) GA/CT/GA: 8574939 (complementary to (converted) bottom strand) CT/GA/GA: 8559411 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1206892002 Total methylated C's in CpG context: 16575220 Total methylated C's in CHG context: 1026274 Total methylated C's in CHH context: 4320799 Total methylated C's in Unknown context: 42248 Total unmethylated C's in CpG context: 137880004 Total unmethylated C's in CHG context: 210169564 Total unmethylated C's in CHH context: 836920141 Total unmethylated C's in Unknown context: 3005737 C methylated in CpG context: 10.7% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.4% Bismark completed in 0d 5h 31m 17s