Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R2_val_2_val_2_val_2.fq.gz

Data processed at 22:10:51 on 2021-01-25


Alignment Stats

Sequence pairs analysed in total 56063348
Paired-end alignments with a unique best hit 34270614
Pairs without alignments under any condition 14832830
Pairs that did not map uniquely 6959904
Genomic sequence context not extractable (edges of chromosomes) 4580

Cytosine Methylation

Total C's analysed 1206892002
Methylated C's in CpG context 16575220
Methylated C's in CHG context 1026274
Methylated C's in CHH context 4320799
Methylated C's in Unknown context 42248
Unmethylated C's in CpG context 137880004
Unmethylated C's in CHG context 210169564
Unmethylated C's in CHH context 836920141
Unmethylated C's in Unknown context 3005737
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8553748 original top strand
CTOT 8577936 complementary to original top strand
CTOB 8574939 complementary to original bottom strand
OB 8559411 original bottom strand

Deduplication

Alignments analysed 34266034
Unique alignments 30199415
Duplicates removed 4066619
Duplicated alignments were found at 3555034 different positions

Cytosine Methylation after Extraction

Total C's analysed 726169635
Methylated C's in CpG context 9757130
Methylated C's in CHG context 610034
Methylated C's in CHH context 2576124
Unmethylated C's in CpG context 82339830
Unmethylated C's in CHG context 125295846
Unmethylated C's in CHH context 505590671
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot