Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_9_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_9_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:40 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 47573665
Paired-end alignments with a unique best hit 28850286
Pairs without alignments under any condition 10440111
Pairs that did not map uniquely 8283268
Genomic sequence context not extractable (edges of chromosomes) 125

Cytosine Methylation

Total C's analysed 1009343213
Methylated C's in CpG context 14341155
Methylated C's in CHG context 839909
Methylated C's in CHH context 3477936
Methylated C's in Unknown context 36395
Unmethylated C's in CpG context 118112411
Unmethylated C's in CHG context 176689558
Unmethylated C's in CHH context 695882244
Unmethylated C's in Unknown context 2439145
Percentage methylation (CpG context) 10.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7236844 original top strand
CTOT 7171624 complementary to original top strand
CTOB 7192356 complementary to original bottom strand
OB 7249337 original bottom strand

Deduplication

Alignments analysed 28850161
Unique alignments 25288521
Duplicates removed 3561640
Duplicated alignments were found at 3131522 different positions

Cytosine Methylation after Extraction

Total C's analysed 583047386
Methylated C's in CpG context 8065728
Methylated C's in CHG context 479515
Methylated C's in CHH context 2006654
Unmethylated C's in CpG context 67237922
Unmethylated C's in CHG context 101006647
Unmethylated C's in CHH context 404250920
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot