Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_8_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_8_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 42336845
Paired-end alignments with a unique best hit 25613542
Pairs without alignments under any condition 9734323
Pairs that did not map uniquely 6988980
Genomic sequence context not extractable (edges of chromosomes) 94

Cytosine Methylation

Total C's analysed 906146238
Methylated C's in CpG context 12408006
Methylated C's in CHG context 784334
Methylated C's in CHH context 3275170
Methylated C's in Unknown context 32964
Unmethylated C's in CpG context 105182054
Unmethylated C's in CHG context 157449282
Unmethylated C's in CHH context 627047392
Unmethylated C's in Unknown context 2202407
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6391563 original top strand
CTOT 6394526 complementary to original top strand
CTOB 6413525 complementary to original bottom strand
OB 6413834 original bottom strand

Deduplication

Alignments analysed 25613448
Unique alignments 22869748
Duplicates removed 2743700
Duplicated alignments were found at 2449204 different positions

Cytosine Methylation after Extraction

Total C's analysed 555061769
Methylated C's in CpG context 7434929
Methylated C's in CHG context 475617
Methylated C's in CHH context 1995391
Unmethylated C's in CpG context 63708290
Unmethylated C's in CHG context 95620397
Unmethylated C's in CHH context 385827145
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot