Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_7_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_7_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 81572749
Paired-end alignments with a unique best hit 49340377
Pairs without alignments under any condition 19347824
Pairs that did not map uniquely 12884548
Genomic sequence context not extractable (edges of chromosomes) 198

Cytosine Methylation

Total C's analysed 1748557586
Methylated C's in CpG context 23524583
Methylated C's in CHG context 1465330
Methylated C's in CHH context 6122809
Methylated C's in Unknown context 59500
Unmethylated C's in CpG context 200474379
Unmethylated C's in CHG context 300747271
Unmethylated C's in CHH context 1216223214
Unmethylated C's in Unknown context 4334330
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 12321508 original top strand
CTOT 12320256 complementary to original top strand
CTOB 12348540 complementary to original bottom strand
OB 12349875 original bottom strand

Deduplication

Alignments analysed 49340179
Unique alignments 43826281
Duplicates removed 5513898
Duplicated alignments were found at 4890647 different positions

Cytosine Methylation after Extraction

Total C's analysed 1078990983
Methylated C's in CpG context 14181698
Methylated C's in CHG context 892953
Methylated C's in CHH context 3753193
Unmethylated C's in CpG context 122189643
Unmethylated C's in CHG context 183848599
Unmethylated C's in CHH context 754124897
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot