Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_6_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_6_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 53174813
Paired-end alignments with a unique best hit 32472179
Pairs without alignments under any condition 12120318
Pairs that did not map uniquely 8582316
Genomic sequence context not extractable (edges of chromosomes) 143

Cytosine Methylation

Total C's analysed 1151670653
Methylated C's in CpG context 15245582
Methylated C's in CHG context 967631
Methylated C's in CHH context 4043361
Methylated C's in Unknown context 38303
Unmethylated C's in CpG context 132527322
Unmethylated C's in CHG context 198821271
Unmethylated C's in CHH context 800065486
Unmethylated C's in Unknown context 2826406
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8112455 original top strand
CTOT 8099104 complementary to original top strand
CTOB 8123747 complementary to original bottom strand
OB 8136730 original bottom strand

Deduplication

Alignments analysed 32472036
Unique alignments 28610892
Duplicates removed 3861144
Duplicated alignments were found at 3417827 different positions

Cytosine Methylation after Extraction

Total C's analysed 699334840
Methylated C's in CpG context 9060292
Methylated C's in CHG context 580883
Methylated C's in CHH context 2442123
Unmethylated C's in CpG context 79624261
Unmethylated C's in CHG context 119729436
Unmethylated C's in CHH context 487897845
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot