Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_5_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_5_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 44083656
Paired-end alignments with a unique best hit 26804023
Pairs without alignments under any condition 10278106
Pairs that did not map uniquely 7001527
Genomic sequence context not extractable (edges of chromosomes) 112

Cytosine Methylation

Total C's analysed 952207866
Methylated C's in CpG context 12628932
Methylated C's in CHG context 797225
Methylated C's in CHH context 3315787
Methylated C's in Unknown context 33109
Unmethylated C's in CpG context 109297758
Unmethylated C's in CHG context 164227970
Unmethylated C's in CHH context 661940194
Unmethylated C's in Unknown context 2328229
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6645834 original top strand
CTOT 6738674 complementary to original top strand
CTOB 6756378 complementary to original bottom strand
OB 6663025 original bottom strand

Deduplication

Alignments analysed 26803911
Unique alignments 23962432
Duplicates removed 2841479
Duplicated alignments were found at 2547583 different positions

Cytosine Methylation after Extraction

Total C's analysed 589669068
Methylated C's in CpG context 7645616
Methylated C's in CHG context 489110
Methylated C's in CHH context 2043470
Unmethylated C's in CpG context 66968289
Unmethylated C's in CHG context 100804689
Unmethylated C's in CHH context 411717894
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot