Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_4_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_4_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 49306248
Paired-end alignments with a unique best hit 29947855
Pairs without alignments under any condition 11369491
Pairs that did not map uniquely 7988902
Genomic sequence context not extractable (edges of chromosomes) 134

Cytosine Methylation

Total C's analysed 1061581694
Methylated C's in CpG context 13679546
Methylated C's in CHG context 850507
Methylated C's in CHH context 3492407
Methylated C's in Unknown context 35439
Unmethylated C's in CpG context 121952808
Unmethylated C's in CHG context 181799911
Unmethylated C's in CHH context 739806515
Unmethylated C's in Unknown context 2616781
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7489798 original top strand
CTOT 7474853 complementary to original top strand
CTOB 7478087 complementary to original bottom strand
OB 7504983 original bottom strand

Deduplication

Alignments analysed 29947721
Unique alignments 26518997
Duplicates removed 3428724
Duplicated alignments were found at 3064526 different positions

Cytosine Methylation after Extraction

Total C's analysed 646265002
Methylated C's in CpG context 8134524
Methylated C's in CHG context 512690
Methylated C's in CHH context 2115791
Unmethylated C's in CpG context 73563281
Unmethylated C's in CHG context 109727960
Unmethylated C's in CHH context 452210756
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot