Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_3_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_3_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 38913609
Paired-end alignments with a unique best hit 23587627
Pairs without alignments under any condition 9049921
Pairs that did not map uniquely 6276061
Genomic sequence context not extractable (edges of chromosomes) 93

Cytosine Methylation

Total C's analysed 836335042
Methylated C's in CpG context 11075871
Methylated C's in CHG context 686982
Methylated C's in CHH context 2878096
Methylated C's in Unknown context 28791
Unmethylated C's in CpG context 96926138
Unmethylated C's in CHG context 144520452
Unmethylated C's in CHH context 580247503
Unmethylated C's in Unknown context 2039558
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5867507 original top strand
CTOT 5901456 complementary to original top strand
CTOB 5918738 complementary to original bottom strand
OB 5899833 original bottom strand

Deduplication

Alignments analysed 23587534
Unique alignments 21186405
Duplicates removed 2401129
Duplicated alignments were found at 2147281 different positions

Cytosine Methylation after Extraction

Total C's analysed 519959348
Methylated C's in CpG context 6749497
Methylated C's in CHG context 422214
Methylated C's in CHH context 1778619
Unmethylated C's in CpG context 59579621
Unmethylated C's in CHG context 89085714
Unmethylated C's in CHH context 362343683
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot