Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_2_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_2_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:39 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 55025914
Paired-end alignments with a unique best hit 33388393
Pairs without alignments under any condition 12972059
Pairs that did not map uniquely 8665462
Genomic sequence context not extractable (edges of chromosomes) 120

Cytosine Methylation

Total C's analysed 1195501862
Methylated C's in CpG context 15540506
Methylated C's in CHG context 983606
Methylated C's in CHH context 4120629
Methylated C's in Unknown context 38661
Unmethylated C's in CpG context 139270636
Unmethylated C's in CHG context 205694628
Unmethylated C's in CHH context 829891857
Unmethylated C's in Unknown context 2941456
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8328453 original top strand
CTOT 8319972 complementary to original top strand
CTOB 8363956 complementary to original bottom strand
OB 8375892 original bottom strand

Deduplication

Alignments analysed 33388273
Unique alignments 29196594
Duplicates removed 4191679
Duplicated alignments were found at 3641246 different positions

Cytosine Methylation after Extraction

Total C's analysed 736774337
Methylated C's in CpG context 9415275
Methylated C's in CHG context 599477
Methylated C's in CHH context 2528365
Unmethylated C's in CpG context 84381751
Unmethylated C's in CHG context 125621587
Unmethylated C's in CHH context 514227882
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot