Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_24_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_24_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 52244045
Paired-end alignments with a unique best hit 32160291
Pairs without alignments under any condition 11633099
Pairs that did not map uniquely 8450655
Genomic sequence context not extractable (edges of chromosomes) 149

Cytosine Methylation

Total C's analysed 1144875434
Methylated C's in CpG context 15276450
Methylated C's in CHG context 973409
Methylated C's in CHH context 4046223
Methylated C's in Unknown context 38191
Unmethylated C's in CpG context 133324942
Unmethylated C's in CHG context 199450694
Unmethylated C's in CHH context 791803716
Unmethylated C's in Unknown context 2772798
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8029544 original top strand
CTOT 8028667 complementary to original top strand
CTOB 8050376 complementary to original bottom strand
OB 8051555 original bottom strand

Deduplication

Alignments analysed 32160142
Unique alignments 28366652
Duplicates removed 3793490
Duplicated alignments were found at 3343763 different positions

Cytosine Methylation after Extraction

Total C's analysed 696432907
Methylated C's in CpG context 9100912
Methylated C's in CHG context 585821
Methylated C's in CHH context 2448615
Unmethylated C's in CpG context 80020781
Unmethylated C's in CHG context 120216217
Unmethylated C's in CHH context 484060561
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot