Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_23_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_23_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 39902509
Paired-end alignments with a unique best hit 24454569
Pairs without alignments under any condition 8843135
Pairs that did not map uniquely 6604805
Genomic sequence context not extractable (edges of chromosomes) 117

Cytosine Methylation

Total C's analysed 864632731
Methylated C's in CpG context 11512912
Methylated C's in CHG context 736417
Methylated C's in CHH context 3067998
Methylated C's in Unknown context 29071
Unmethylated C's in CpG context 101057007
Unmethylated C's in CHG context 150569820
Unmethylated C's in CHH context 597688577
Unmethylated C's in Unknown context 2080551
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6128719 original top strand
CTOT 6076316 complementary to original top strand
CTOB 6097096 complementary to original bottom strand
OB 6152321 original bottom strand

Deduplication

Alignments analysed 24454452
Unique alignments 21664232
Duplicates removed 2790220
Duplicated alignments were found at 2480381 different positions

Cytosine Methylation after Extraction

Total C's analysed 522829732
Methylated C's in CpG context 6800694
Methylated C's in CHG context 439937
Methylated C's in CHH context 1843326
Unmethylated C's in CpG context 60321590
Unmethylated C's in CHG context 90190580
Unmethylated C's in CHH context 363233605
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot