Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_22_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_22_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 47019646
Paired-end alignments with a unique best hit 28927483
Pairs without alignments under any condition 10487326
Pairs that did not map uniquely 7604837
Genomic sequence context not extractable (edges of chromosomes) 126

Cytosine Methylation

Total C's analysed 1030634534
Methylated C's in CpG context 13865332
Methylated C's in CHG context 888058
Methylated C's in CHH context 3664744
Methylated C's in Unknown context 33271
Unmethylated C's in CpG context 120629163
Unmethylated C's in CHG context 180106800
Unmethylated C's in CHH context 711480437
Unmethylated C's in Unknown context 2469594
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7198503 original top strand
CTOT 7245331 complementary to original top strand
CTOB 7265217 complementary to original bottom strand
OB 7218306 original bottom strand

Deduplication

Alignments analysed 28927357
Unique alignments 25586704
Duplicates removed 3340653
Duplicated alignments were found at 2954476 different positions

Cytosine Methylation after Extraction

Total C's analysed 629662971
Methylated C's in CpG context 8270805
Methylated C's in CHG context 535234
Methylated C's in CHH context 2223280
Unmethylated C's in CpG context 72627174
Unmethylated C's in CHG context 108957659
Unmethylated C's in CHH context 437048819
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot