Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_21_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_21_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 50457301
Paired-end alignments with a unique best hit 30628245
Pairs without alignments under any condition 11802929
Pairs that did not map uniquely 8026127
Genomic sequence context not extractable (edges of chromosomes) 99

Cytosine Methylation

Total C's analysed 1084406446
Methylated C's in CpG context 14616250
Methylated C's in CHG context 914023
Methylated C's in CHH context 3783477
Methylated C's in Unknown context 36538
Unmethylated C's in CpG context 125426401
Unmethylated C's in CHG context 187840703
Unmethylated C's in CHH context 751825592
Unmethylated C's in Unknown context 2640043
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7652272 original top strand
CTOT 7641249 complementary to original top strand
CTOB 7661942 complementary to original bottom strand
OB 7672683 original bottom strand

Deduplication

Alignments analysed 30628146
Unique alignments 27142162
Duplicates removed 3485984
Duplicated alignments were found at 3104659 different positions

Cytosine Methylation after Extraction

Total C's analysed 671280147
Methylated C's in CpG context 8813988
Methylated C's in CHG context 558542
Methylated C's in CHH context 2324612
Unmethylated C's in CpG context 76705613
Unmethylated C's in CHG context 115125144
Unmethylated C's in CHH context 467752248
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot