Bismark report for: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Hawes/data/Cg-roslin/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53390071 Number of paired-end alignments with a unique best hit: 32235145 Mapping efficiency: 60.4% Sequence pairs with no alignments under any condition: 12794800 Sequence pairs did not map uniquely: 8360126 Sequence pairs which were discarded because genomic sequence could not be extracted: 150 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7753017 ((converted) top strand) GA/CT/CT: 8341465 (complementary to (converted) top strand) GA/CT/GA: 8364216 (complementary to (converted) bottom strand) CT/GA/GA: 7776297 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1143360567 Total methylated C's in CpG context: 15227780 Total methylated C's in CHG context: 982190 Total methylated C's in CHH context: 4048786 Total methylated C's in Unknown context: 42158 Total unmethylated C's in CpG context: 132587393 Total unmethylated C's in CHG context: 198046482 Total unmethylated C's in CHH context: 792467936 Total unmethylated C's in Unknown context: 2735560 C methylated in CpG context: 10.3% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.5% Bismark completed in 0d 5h 6m 47s