Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_20_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 53390071
Paired-end alignments with a unique best hit 32235145
Pairs without alignments under any condition 12794800
Pairs that did not map uniquely 8360126
Genomic sequence context not extractable (edges of chromosomes) 150

Cytosine Methylation

Total C's analysed 1143360567
Methylated C's in CpG context 15227780
Methylated C's in CHG context 982190
Methylated C's in CHH context 4048786
Methylated C's in Unknown context 42158
Unmethylated C's in CpG context 132587393
Unmethylated C's in CHG context 198046482
Unmethylated C's in CHH context 792467936
Unmethylated C's in Unknown context 2735560
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7753017 original top strand
CTOT 8341465 complementary to original top strand
CTOB 8364216 complementary to original bottom strand
OB 7776297 original bottom strand

Deduplication

Alignments analysed 32234995
Unique alignments 28396232
Duplicates removed 3838763
Duplicated alignments were found at 3390844 different positions

Cytosine Methylation after Extraction

Total C's analysed 705659428
Methylated C's in CpG context 9167500
Methylated C's in CHG context 602562
Methylated C's in CHH context 2495442
Unmethylated C's in CpG context 80847798
Unmethylated C's in CHG context 121093094
Unmethylated C's in CHH context 491453032
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot