Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_1_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_1_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 50993451
Paired-end alignments with a unique best hit 31434721
Pairs without alignments under any condition 11134052
Pairs that did not map uniquely 8424678
Genomic sequence context not extractable (edges of chromosomes) 115

Cytosine Methylation

Total C's analysed 1113134972
Methylated C's in CpG context 13763919
Methylated C's in CHG context 897841
Methylated C's in CHH context 3720045
Methylated C's in Unknown context 35255
Unmethylated C's in CpG context 131347662
Unmethylated C's in CHG context 194126289
Unmethylated C's in CHH context 769279216
Unmethylated C's in Unknown context 2644308
Percentage methylation (CpG context) 9.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7891335 original top strand
CTOT 7795639 complementary to original top strand
CTOB 7818927 complementary to original bottom strand
OB 7928705 original bottom strand

Deduplication

Alignments analysed 31434606
Unique alignments 27517298
Duplicates removed 3917308
Duplicated alignments were found at 3402879 different positions

Cytosine Methylation after Extraction

Total C's analysed 656669587
Methylated C's in CpG context 7963596
Methylated C's in CHG context 524106
Methylated C's in CHH context 2189730
Unmethylated C's in CpG context 76099250
Unmethylated C's in CHG context 113434668
Unmethylated C's in CHH context 456458237
Percentage methylation (CpG context) 9.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot