Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_19_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_19_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:38 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 48641567
Paired-end alignments with a unique best hit 29709816
Pairs without alignments under any condition 11031854
Pairs that did not map uniquely 7899897
Genomic sequence context not extractable (edges of chromosomes) 136

Cytosine Methylation

Total C's analysed 1049309911
Methylated C's in CpG context 13871848
Methylated C's in CHG context 885885
Methylated C's in CHH context 3714128
Methylated C's in Unknown context 34625
Unmethylated C's in CpG context 121771359
Unmethylated C's in CHG context 182184732
Unmethylated C's in CHH context 726881959
Unmethylated C's in Unknown context 2544542
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7407406 original top strand
CTOT 7419329 complementary to original top strand
CTOB 7443202 complementary to original bottom strand
OB 7439743 original bottom strand

Deduplication

Alignments analysed 29709680
Unique alignments 26595143
Duplicates removed 3114537
Duplicated alignments were found at 2785014 different positions

Cytosine Methylation after Extraction

Total C's analysed 651191131
Methylated C's in CpG context 8400203
Methylated C's in CHG context 541481
Methylated C's in CHH context 2285729
Unmethylated C's in CpG context 74612966
Unmethylated C's in CHG context 111985513
Unmethylated C's in CHH context 453365239
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot