Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_18_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_18_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:37 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 50943423
Paired-end alignments with a unique best hit 31468183
Pairs without alignments under any condition 11256355
Pairs that did not map uniquely 8218885
Genomic sequence context not extractable (edges of chromosomes) 180

Cytosine Methylation

Total C's analysed 1116445364
Methylated C's in CpG context 15247444
Methylated C's in CHG context 959631
Methylated C's in CHH context 3950285
Methylated C's in Unknown context 36698
Unmethylated C's in CpG context 130500810
Unmethylated C's in CHG context 195471883
Unmethylated C's in CHH context 770315311
Unmethylated C's in Unknown context 2666148
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7873817 original top strand
CTOT 7843653 complementary to original top strand
CTOB 7858680 complementary to original bottom strand
OB 7891853 original bottom strand

Deduplication

Alignments analysed 31468003
Unique alignments 27964963
Duplicates removed 3503040
Duplicated alignments were found at 3091843 different positions

Cytosine Methylation after Extraction

Total C's analysed 680992148
Methylated C's in CpG context 9100011
Methylated C's in CHG context 578143
Methylated C's in CHH context 2394879
Unmethylated C's in CpG context 78273455
Unmethylated C's in CHG context 118029388
Unmethylated C's in CHH context 472616272
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot