Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_17_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_17_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:37 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 57429743
Paired-end alignments with a unique best hit 35252677
Pairs without alignments under any condition 12521081
Pairs that did not map uniquely 9655985
Genomic sequence context not extractable (edges of chromosomes) 164

Cytosine Methylation

Total C's analysed 1257976076
Methylated C's in CpG context 16913211
Methylated C's in CHG context 1080223
Methylated C's in CHH context 4465430
Methylated C's in Unknown context 41529
Unmethylated C's in CpG context 150059765
Unmethylated C's in CHG context 220480977
Unmethylated C's in CHH context 864976470
Unmethylated C's in Unknown context 2971793
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8773352 original top strand
CTOT 8819837 complementary to original top strand
CTOB 8852263 complementary to original bottom strand
OB 8807061 original bottom strand

Deduplication

Alignments analysed 35252513
Unique alignments 30818320
Duplicates removed 4434193
Duplicated alignments were found at 3792438 different positions

Cytosine Methylation after Extraction

Total C's analysed 753684584
Methylated C's in CpG context 9962303
Methylated C's in CHG context 640339
Methylated C's in CHH context 2667807
Unmethylated C's in CpG context 87776962
Unmethylated C's in CHG context 130678592
Unmethylated C's in CHH context 521958581
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot