Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_16_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_16_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:37 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 50386602
Paired-end alignments with a unique best hit 30924916
Pairs without alignments under any condition 10955437
Pairs that did not map uniquely 8506249
Genomic sequence context not extractable (edges of chromosomes) 134

Cytosine Methylation

Total C's analysed 1084479570
Methylated C's in CpG context 14831216
Methylated C's in CHG context 907536
Methylated C's in CHH context 3766929
Methylated C's in Unknown context 38054
Unmethylated C's in CpG context 126448721
Unmethylated C's in CHG context 188794776
Unmethylated C's in CHH context 749730392
Unmethylated C's in Unknown context 2598022
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7714679 original top strand
CTOT 7730844 complementary to original top strand
CTOB 7749791 complementary to original bottom strand
OB 7729468 original bottom strand

Deduplication

Alignments analysed 30924782
Unique alignments 27159851
Duplicates removed 3764931
Duplicated alignments were found at 3310491 different positions

Cytosine Methylation after Extraction

Total C's analysed 633174381
Methylated C's in CpG context 8466508
Methylated C's in CHG context 523848
Methylated C's in CHH context 2191253
Unmethylated C's in CpG context 72724239
Unmethylated C's in CHG context 109136529
Unmethylated C's in CHH context 440132004
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot