Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_15_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_15_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:37 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 58435477
Paired-end alignments with a unique best hit 35609199
Pairs without alignments under any condition 13456383
Pairs that did not map uniquely 9369895
Genomic sequence context not extractable (edges of chromosomes) 206

Cytosine Methylation

Total C's analysed 1260311301
Methylated C's in CpG context 18139805
Methylated C's in CHG context 1085026
Methylated C's in CHH context 4528243
Methylated C's in Unknown context 45183
Unmethylated C's in CpG context 144086822
Unmethylated C's in CHG context 217607400
Unmethylated C's in CHH context 874864005
Unmethylated C's in Unknown context 3099158
Percentage methylation (CpG context) 11.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8900271 original top strand
CTOT 8885262 complementary to original top strand
CTOB 8907048 complementary to original bottom strand
OB 8916412 original bottom strand

Deduplication

Alignments analysed 35608993
Unique alignments 31449079
Duplicates removed 4159914
Duplicated alignments were found at 3690556 different positions

Cytosine Methylation after Extraction

Total C's analysed 769374088
Methylated C's in CpG context 10807738
Methylated C's in CHG context 654007
Methylated C's in CHH context 2746440
Unmethylated C's in CpG context 86998035
Unmethylated C's in CHG context 131603656
Unmethylated C's in CHH context 536564212
Percentage methylation (CpG context) 11.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot