Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_14_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_14_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:37 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 36340861
Paired-end alignments with a unique best hit 22341478
Pairs without alignments under any condition 8183804
Pairs that did not map uniquely 5815579
Genomic sequence context not extractable (edges of chromosomes) 79

Cytosine Methylation

Total C's analysed 788907403
Methylated C's in CpG context 11967003
Methylated C's in CHG context 694010
Methylated C's in CHH context 2844733
Methylated C's in Unknown context 29476
Unmethylated C's in CpG context 89548490
Unmethylated C's in CHG context 136421293
Unmethylated C's in CHH context 547431874
Unmethylated C's in Unknown context 1911810
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5552803 original top strand
CTOT 5596286 complementary to original top strand
CTOB 5618114 complementary to original bottom strand
OB 5574196 original bottom strand

Deduplication

Alignments analysed 22341399
Unique alignments 20014759
Duplicates removed 2326640
Duplicated alignments were found at 2094266 different positions

Cytosine Methylation after Extraction

Total C's analysed 487571051
Methylated C's in CpG context 7212539
Methylated C's in CHG context 424318
Methylated C's in CHH context 1747285
Unmethylated C's in CpG context 54807921
Unmethylated C's in CHG context 83542001
Unmethylated C's in CHH context 339836987
Percentage methylation (CpG context) 11.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot