Bismark report for: /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R2_val_2_val_2_val_2.fq.gz (version: v0.22.3) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/yaaminiv/Hawes/data/Cg-roslin/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 53303436 Number of paired-end alignments with a unique best hit: 32491207 Mapping efficiency: 61.0% Sequence pairs with no alignments under any condition: 12418721 Sequence pairs did not map uniquely: 8393508 Sequence pairs which were discarded because genomic sequence could not be extracted: 185 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8111596 ((converted) top strand) GA/CT/CT: 8117046 (complementary to (converted) top strand) GA/CT/GA: 8130289 (complementary to (converted) bottom strand) CT/GA/GA: 8132091 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1155305305 Total methylated C's in CpG context: 15463701 Total methylated C's in CHG context: 978549 Total methylated C's in CHH context: 4080737 Total methylated C's in Unknown context: 38975 Total unmethylated C's in CpG context: 133418976 Total unmethylated C's in CHG context: 199756716 Total unmethylated C's in CHH context: 801606626 Total unmethylated C's in Unknown context: 2815212 C methylated in CpG context: 10.4% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.4% Bismark completed in 0d 5h 9m 31s