Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_13_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:36 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 53303436
Paired-end alignments with a unique best hit 32491207
Pairs without alignments under any condition 12418721
Pairs that did not map uniquely 8393508
Genomic sequence context not extractable (edges of chromosomes) 185

Cytosine Methylation

Total C's analysed 1155305305
Methylated C's in CpG context 15463701
Methylated C's in CHG context 978549
Methylated C's in CHH context 4080737
Methylated C's in Unknown context 38975
Unmethylated C's in CpG context 133418976
Unmethylated C's in CHG context 199756716
Unmethylated C's in CHH context 801606626
Unmethylated C's in Unknown context 2815212
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8111596 original top strand
CTOT 8117046 complementary to original top strand
CTOB 8130289 complementary to original bottom strand
OB 8132091 original bottom strand

Deduplication

Alignments analysed 32491022
Unique alignments 28613670
Duplicates removed 3877352
Duplicated alignments were found at 3421180 different positions

Cytosine Methylation after Extraction

Total C's analysed 709843283
Methylated C's in CpG context 9295422
Methylated C's in CHG context 593607
Methylated C's in CHH context 2492145
Unmethylated C's in CpG context 80852274
Unmethylated C's in CHG context 121573406
Unmethylated C's in CHH context 495036429
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot