Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_12_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_12_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:36 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 46849571
Paired-end alignments with a unique best hit 28579240
Pairs without alignments under any condition 10827059
Pairs that did not map uniquely 7443272
Genomic sequence context not extractable (edges of chromosomes) 114

Cytosine Methylation

Total C's analysed 1002502546
Methylated C's in CpG context 12372696
Methylated C's in CHG context 794788
Methylated C's in CHH context 3327340
Methylated C's in Unknown context 32248
Unmethylated C's in CpG context 113572348
Unmethylated C's in CHG context 168647208
Unmethylated C's in CHH context 703788166
Unmethylated C's in Unknown context 2522025
Percentage methylation (CpG context) 9.8%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7112038 original top strand
CTOT 7173632 complementary to original top strand
CTOB 7169686 complementary to original bottom strand
OB 7123770 original bottom strand

Deduplication

Alignments analysed 28579126
Unique alignments 25427638
Duplicates removed 3151488
Duplicated alignments were found at 2832721 different positions

Cytosine Methylation after Extraction

Total C's analysed 610362234
Methylated C's in CpG context 7330260
Methylated C's in CHG context 477710
Methylated C's in CHH context 2009270
Unmethylated C's in CpG context 68536895
Unmethylated C's in CHG context 101729911
Unmethylated C's in CHH context 430278188
Percentage methylation (CpG context) 9.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot