Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_11_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:36 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 56063348
Paired-end alignments with a unique best hit 33950348
Pairs without alignments under any condition 13018208
Pairs that did not map uniquely 9094792
Genomic sequence context not extractable (edges of chromosomes) 112

Cytosine Methylation

Total C's analysed 1200110470
Methylated C's in CpG context 16333777
Methylated C's in CHG context 1009426
Methylated C's in CHH context 4229214
Methylated C's in Unknown context 42306
Unmethylated C's in CpG context 139879266
Unmethylated C's in CHG context 208783817
Unmethylated C's in CHH context 829874970
Unmethylated C's in Unknown context 2928000
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8463459 original top strand
CTOT 8484562 complementary to original top strand
CTOB 8509872 complementary to original bottom strand
OB 8492343 original bottom strand

Deduplication

Alignments analysed 33950236
Unique alignments 29999792
Duplicates removed 3950444
Duplicated alignments were found at 3485585 different positions

Cytosine Methylation after Extraction

Total C's analysed 726425704
Methylated C's in CpG context 9664112
Methylated C's in CHG context 603679
Methylated C's in CHH context 2547933
Unmethylated C's in CpG context 83415513
Unmethylated C's in CHG context 125099513
Unmethylated C's in CHH context 505094954
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot