Bismark

Bismark Processing Report

/gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_10_R1_val_1_val_1_val_1.fq.gz and /gscratch/scrubbed/yaaminiv/Hawes/analyses/trimgalore-2/poly-G/zr3644_10_R2_val_2_val_2_val_2.fq.gz

Data processed at 21:40:36 on 2021-03-10


Alignment Stats

Sequence pairs analysed in total 51902712
Paired-end alignments with a unique best hit 31449874
Pairs without alignments under any condition 12046900
Pairs that did not map uniquely 8405938
Genomic sequence context not extractable (edges of chromosomes) 103

Cytosine Methylation

Total C's analysed 1115773727
Methylated C's in CpG context 15618471
Methylated C's in CHG context 940915
Methylated C's in CHH context 3919549
Methylated C's in Unknown context 38912
Unmethylated C's in CpG context 130395725
Unmethylated C's in CHG context 194787000
Unmethylated C's in CHH context 770112067
Unmethylated C's in Unknown context 2693516
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 7880637 original top strand
CTOT 7807411 complementary to original top strand
CTOB 7846348 complementary to original bottom strand
OB 7915375 original bottom strand

Deduplication

Alignments analysed 31449771
Unique alignments 27724085
Duplicates removed 3725686
Duplicated alignments were found at 3277491 different positions

Cytosine Methylation after Extraction

Total C's analysed 673820747
Methylated C's in CpG context 9214054
Methylated C's in CHG context 561215
Methylated C's in CHH context 2356777
Unmethylated C's in CpG context 77490239
Unmethylated C's in CHG context 116411583
Unmethylated C's in CHH context 467786879
Percentage methylation (CpG context) 10.6%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot